Replication of bacteriophage DNA, II. Structure of replicating DNA of phage lambda.

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The Structure and Function of the DNA from Bacteriophage Lambda

The position and orientation of genes in lambda and lambda dg DNA are described. The position of six genes located in the right half of isolated lambda DNA was found to be -(N, i(lambda))--O-P---Q-R-(right end of DNA), which is their order on the genetic map of the vegetative phage. The order of the three genes of the galactose operon (k, t, and e) located in the left half of lambda dg DNA was ...

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The nucleotide sequence of the O gene in bacteriophage lambda DNA is presented. According to two possible initiator codons, the primary structure of the O protein deduced from the DNA sequence consists of 278 or 299 amino acid residues. Structure and function of the O protein--one of the two phage initiator proteins for lambda DNA replication--are discussed in the light of a secondary structure...

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Four T7 products, DNA polymerase, gene 4 protein, RNA polymerase, and DNA binding protein, have been purified from phage-infected cells. It has been previously shown (Hinkle, D. C., and Richardson, C. C. (1975) J. Biol. Chem 250, 5523-5529; Kolodner, R., and Richardson, C, C. (1978) J. Biol. Chem 253, 574-584) that two T7 products, DNA polymerase and gene 4 protein, catalyze extensive synthesis...

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ژورنال

عنوان ژورنال: Proceedings of the National Academy of Sciences

سال: 1968

ISSN: 0027-8424,1091-6490

DOI: 10.1073/pnas.60.3.861